background:

surface modification PCL-GO nanofiber increase
immobilization of biomolecules and specific activity of the enzyme Horseradish
peroxidase.

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Method:

Graphene oxide (GO) was synthesized from graphite
powders and the morphologies of Graphene oxide was examined with an AFM and TEM
operated at 200 kV. than 1% GO with PCL electrorised in 18KV.The surface of
nanofiber PCL-GO hydrolyzed with NaOH 5 M and NaOH 0.25 M and chlorine acetic
acid 0.125 M and then 200 µg/ml enzyme Horseradish peroxidase immobilized with
using of EDC & NHS in a surface of hydrolized nanofiber and detected the
presence of TMB substrate in 450 nm.FTIR was used to investigate the chemical
functional groups for immobilization of BSA protein

Result:

the morphologies analysis of Graphene oxide indicated
reduced GO sheets without obvious agglomeration. In addition, the thickness of
the obtained GO nano sheets was found to be 2 nm by AFM the chemical
modification of the nanofiber surface resulted in increased protein
immobilization. So that immobilization of enzyme Horseradish peroxidase
indicated increase specific activity and further stability against the changes
in the configuration of the enzyme.

 the FTIR
analysis confirmed the successful immobilization with the method of covalent
attachment the biomolecules on the surfaces of the nanostructures. 

conclusions:

Surface immobilized nanostructures with bioactive
molecules have provided great opportunities for developing diagnostic sensors,
bio-probes, and biomedical devices. the results indicated that the hydrolyzed
surface was modified by further stability against the changes in the
configuration and also increase the specific activity of the enzyme and
hydrolyzing with NaOH 5 M confirmed the successful immobilization of the
biomolecules on the surface of the PCL-GO fibrous scaffolds. keywords: PCL_GO , enzyme Horseradish peroxidase , surface
modification