Melanomas is a type of fly that belongs to the Drosophila family under the Phylum Arthropod. Drosophila Melanomas is also known as the Common fruit fly or Vinegar fly. This type of fly is a very popular organism for genetics research for many different reasons. The flies are small and easy to care for in the laboratory and only have 4 pairs of chromosomes. Drosophila Melanomas have short life cycle about 30 days long and females are fecund and can lay hundreds of fertilized eggs during their brief life cycle.
The larger amount of offspring allow for statistical analysis to be easy and reliable (Drosophila). Also the fact that their embryo grow outside the body allows for study of the organism at every stage. Another reason they are very popular for use Is mutations can be easily targeted to specific genes. The life cycle of the flies Is very simple and duration varies with according to temperature which is a characteristic of all cold- blooded Insects (Sanitarian). The first stage of the cycle is egg form each egg is measured to be around 0. Mm a female can lay up to 400 eggs in favorable conditions. After hatch he Drosophila Melanomas goes through three instars stages as larvae. When the larvae has molted twice, It will have reached the third instars. The third instars now goes through a four day metamorphosis where it encapsulates itself inside a premium and arise as adults (Cassandra). For the experiment, a pair reciprocal did- hybrid cross was set up. My cross was between ebony (black body) males and vestigial (deformed, useless wings) females and the reciprocal cross between ebony females and vestigial males.
The purpose of this lab was to Investigate whether the genes being tested were sex linked or autocross linked genes. Another purpose of the lab was to determine whether the mutation was dominant or recessive between the genes. The way to asses that was to trace the heredity. Methods and Materials: To complete this experiment we used homozygous ebony and vestigial flies. We prepared the first medium between the two flies to obtain the reciprocal of the first cross. Provided to use in the lab were two fresh milk bottles.
We added ml of Drosophila medium flakes (Carolina Biological) to each of them. The Drosophila medium flakes are the food which the larvae will eat during their growth period. Then add ml of distilled water to complete the food and place 10 grains of yeast on the top which will be the food for the future adult flies. Once the medium was finished we labeled the bottle with the sex and phenotype (ebony and vestigial) of the flies along with each lab partners name. We collected the 10 ebony virgin and 10 non-vulgar which was Instructed to do also for the vestigial totaling at 40 flies.
Flyway was used as anesthesia which we placed Inside the vial for 1 to 2 minutes to transfer and count the flies. Once the flies were put out to verify sex and phenotype and to mount now many we put teen on a went card, Ana uses a microscope to get a netter look. Once flies were sorted properly, we then placed those flies into a bottle making sure we sat the bottle on its side so until the flies woke up so they would not get stuck in medium. To keep the flies in the veil we used a sponge plug which also allowed for air to come through.
To produce an IF generation we had to let the flies mate and lay eggs in the first cross and once the flies were done we would remove the adult flies and only keep the offspring from the Fl cross. Once the offspring hatched, we could cross them which would give way to the IF cross. Always make sure to label your bottles so you could identified the Fl cross from the IF cross and show the genotypes of the original parents. Results: In Bottle 1, Parental ebony males crossed with vestigial females produced an Fl generation that expressed only the wild type allele.