In the laboratory, one can directly test the effects of glutathione even to a bacterial strain that is not capable of glutathione biosynthesis, as already exhibited in part from the study conducted by Grossowicz in 1947. The process for conducting such a test must be carefully planned in consideration of the nature of the media to be used in order to come up with a more definitive result. Hence, a proposed test protocol is as follows: 1.
Prepare two plates of media, one having minimal media while the other having minimal media with the addition of glutathione. 2. Inoculate the two plates with staphylococcus aureus coming from the same stock preparation. 3. Incubate and allow to grow for four days, under room temperature; counting cell populations for both plates in six hour intervals. 4. Afterwards, compare the trends and shifts in terms of colony counts, expectedly arriving at a greater value from the plate with added glutathione.
This protocol is focused upon establishing the anti oxidant effect of glutathione on Staphylococcus aureus, through the addition of glutathione in a of the setups. Given that glutathione is considered to be effective against the effects of free radicals such as reactive oxygen species along with toxins produced during metabolism, which of course further increases as the colony ages. It is expected that a greater number will survive through the stationary and death phases if glutathione is present as compared to the colony wherein glutathione is not added.
Therefore, through this protocol, the protective effect of glutathione will be exhibited by maintaining survivability in the presence of toxins and free radicals. References Chesney, J. A. , Eaton, J. W. , and Mahoney, J. R. (1996). Bacterial Glutathione: a Sacrificial Defense Against Chlorine Compounds. Journal of Bacteriology, 178(7), 2131 – 2135. Retrieved June 5, 2009 from http://jb. asm. org/cgi/reprint/178/7/2131. pdf. Copley, S. D. and Dhillon, J.
K. (2002). Lateral Gene Transfer and Parallel Evolution in the History of Glutathione Biosynthesis Genes. Genome Biology, 3(5), 1 – 16. Retrieved June 5, 2009 from http://www. pubmedcentral. nih. gov/picrender. fcgi? artid=115227&blobtype=pdf. Grossowicz, N. (1947). Glutamine, an Anti Metabolite for Staphylococcus aureus. Journal of Biological Chemistry, 729 – 736. Retrieved June 5, 2009 from http://www. jbc. org/cgi/ reprint/173/2/729. pdf.